Welcome to fokve | Welcome My Forum

[Hoca]

View eBook​

Messenger ribonucleic acid (mRNA) is a single-stranded molecule of RNA that carries the instructions from DNA to create specific proteins. The main applications for mRNA include immunotherapy, protein replacement therapy, genvomic editing, and regenerative medicine therapy, and notably vaccines. Lipid nanoparticles (LNPs) are the most common drug delivery systems to encapsulate, protect, and transport mRNA. UV-Vis spectroscopy is a frequently used analytical method to determine mRNA concentration; however, quantitative concentration determination for encapsulated mRNA is difficult due to UV light scattering from the LNP.

The CTech

SoloVPER System uses a variable pathlength method to measure concentration, with a light scattering correction algorithm to address the issue presented by scattering from LNPs. This publication demonstrates how the SoloVPE System was used to measure the total mRNA concentration after LNP encapsulation. The method was developed and qualified by assessing the specificity, repeatability, intermediate precision, linearity, and accuracy of the concentration measurements. The direct measurement of mRNA content using the CTech SoloVPE System passed all acceptance criteria, demonstrating the reliability of the variable pathlength method.

The post Revolutionizing mRNA Concentration Measurement with Variable Pathlength Spectroscopy appeared first on GEN - Genetic Engineering and Biotechnology News.